中医学报Issue(4)：536-538,3.DOI:10.16368/j.issn.1674-8999.2015.04.185
姜黄素调控β-分泌酶基因表达抑制老年性痴呆大鼠Aβ蛋白生成的机制
Mechanism on the Restrain of AβProtein Generated of Senile Dementia Rats by Regulating β-Secretase Gene Expression of Curcumin
摘要
Abstract
Objective:To explore the effect of genetic expression of hippocampus' β-APP and BACE1 by curcumin. Methods:Injecting the right hippocampus by Aβ1-40,preparing a model of AD rats,intraperitoneal injection of curcumin,using ELISA to detect the content of Aβ in serum and hippocampus,immune histochemical method to detect the hippocampus neuron inβ-APP protein expression,rt-pcr method to detect beta APP and BACE1mRNA expression in hippocampus. Results:Aβ had been remarkable rise,β-APP expression of protein,β-APPmRNA and BACE1mRNA expression remarkable increase after the molding. Comparing the model group,Curcumin in high dose group,low dose group of beta APP protein expression,beta APPmRNA and significantly lower BACE1mRNA,difference had statistical significance(P<0. 05). Conclusion:Curcumin can interdict β-APP splitting decomposition,Inhibition of Aβformation and deposition in the brain by regulating BACE1 mRNA,therefore reduce the toxic effect of nerve cell.关键词
姜黄素/老年性痴呆/Aβ蛋白/β-淀粉样前体蛋白/β-分泌酶/大鼠Key words
curcumin/senile dementia/Aβ Protein/β-amyloid precusor protein/β-secretase/rat分类
医药卫生引用本文复制引用
韩玉生,侯志涛,纪显玥,李树学,史之茂,徐强,周忠光..姜黄素调控β-分泌酶基因表达抑制老年性痴呆大鼠Aβ蛋白生成的机制[J].中医学报,2015,(4):536-538,3.基金项目
黑龙江省中医药管理局科研项目 （）
