化工进展Issue(6)：1539-1544,6.DOI:10.3969/j.issn.1000-6613.2014.06.030
基于果糖与葡萄糖不同混合比例的丙酮丁醇发酵
Acetone-butanol-ethanol fermentation using mixture of glucose and fructose with different portions
摘要
Abstract
Batch acetone-butanol-ethanol (ABE) fermentations from various substrates were briefly introduced. Limiting factors,such as low fructose utilization and butanol production,were involved in batch ABE fermentation by Clostridium acetobutylicum using 55 g/L mixture of glucose and fructose (1∶4) as substrates to stimulate the hydrolysate of Jerusalem artichoke tubers. Then the effect of fructose and glucose with different portions(1∶4 to 1∶2,2∶3,3∶2 and 3∶1)on the performance of batch ABE fermentation was studied. The results showed that the fermentation time was shortened from 76 h to 48 h as the portion increased,and the cell growth with the max OD620 was enhanced from 2.1 to 4.3. The fermentation gave the best metabolic activity of fructose utilization with the glucose/fructose portion of 1∶2,correspondingly,9.7 g/L and 16.0 g/L production of butanol and ABE were produced with only 2.1 g/L residual sugars,resulting in improved fructose utilization efficiency as high as 95.03%.关键词
生物燃料/丙酮丁醇梭菌/丙酮丁醇发酵/混合糖/果糖利用效率Key words
biofuel/Clostridium acetobutylicum/acetone-butanol-ethanol fermentation/mixed sugars/fructose utilization efficiency分类
化学化工引用本文复制引用
吴又多,付友思,齐高相,陈丽杰,白凤武..基于果糖与葡萄糖不同混合比例的丙酮丁醇发酵[J].化工进展,2014,(6):1539-1544,6.基金项目
国家高技术研究发展计划（2011AA02A208、2012AA021205）及国家自然科学基金（21376044）项目。 （）
