化工进展Issue(9)：2415-2420,6.DOI:10.3969/j.issn.1000-6613.2014.09.030
原生质体诱变提高亚麻刺盘孢ST对底物DHEA的耐受性和转化率
Improvement of DHEA tolerance and conversion efficiency of Colletotrichum lini ST by protpplast mutagenesis
摘要
Abstract
The aims of this research were to isolate a Colletotrichum lini strain with higher DHEA tolerance and conversion efficiency. We utilized C. lini ST as the original strain and investigated the best conditions of protoplast release and regeneration for strain ST. Then the protoplasts of C. lini ST were treated by plasma irradiation and a positive mutant strain ST-0317 with high DHEA concentration tolerance and good genetic stability was obtained. At DHEA concentration of 10g/L,the 7α, 15α-diOH-DHEA molar yield of ST-0317 was increased to 36.9%,with an improvement of 50%compared with the original strain. These results were beneficial for producing 7α,15α-diOH-DHEA through this productive mutant.关键词
亚麻刺盘孢/原生质体/去氢表雄酮/7α,15α-diOH-DHEA/等离子诱变Key words
Colletotrichum lini/protoplasts/dehydroepiandrosterone/7-alpha-15-alpha-double hydroxy dehydroepiandrosterone/mutation分类
生物科学引用本文复制引用
李恒,吴燕,魏利莎,李会,张晓梅,史劲松,许正宏..原生质体诱变提高亚麻刺盘孢ST对底物DHEA的耐受性和转化率[J].化工进展,2014,(9):2415-2420,6.基金项目
国家高技术研究发展计划（2011AA02A211）、国家自然科学基金（21206055）及江苏省自然科学基金（BK2012127）项目。 （）
