化工进展Issue(9)：2425-2431,7.DOI:10.3969/j.issn.1000-6613.2014.09.032
酶法动态动力学拆分制备R-（-）-乙酰基邻氯扁桃酸
R-(-)-acetyl-o-mandelic acid preparation via enzymatic dynamic kinetic resolution
摘要
Abstract
In this study,asymmetric hydrolysis of acetyl chloromandelic acid was catalyzed by Pseudomonas sp. ECU1011,and the S-(-)-chloromandelic acid in the resolution mixture was then racemerized using the mutated mandelate racemase and fed back into the hydrolysis reaction after acylation , realizing enzymatic dynamic kinetic resolution for preparation of R-(-)-acetyl chloromandelic acid. Firstly,the conditions for enzymatic resolution,separation of resolution mixture, and enzymatic racemization were optimized. Subsequently,dynamic kinetic resolution of acetyl chloromandelic acid was conducted under the optimal conditions,obtaining optically pure R-(-)-acetyl chloromandelic acid with final yield of 80%. The conditions for enzymatic resolution,chloromandelic acid recycling and racemization were optimized in this study,leading to preliminary establishment of the dynamic kinetic resolution process for R-(-)-acetyl chloromandelic acid preparation,with guiding significance for future industrial applications.关键词
酶法动态动力学拆分/脂肪酶Pseudomonas sp. ECU1011/扁桃酸消旋酶/乙酰基邻氯扁桃酸/邻氯扁桃酸Key words
enzymatic dynamic kinetic resolution/Pseudomonas sp. ECU1011 lipase/mandelate racemase/R-(-)-acetyl-o-mandelic acid/chloromandelic acid分类
化学化工引用本文复制引用
沈萨萨,姜灵,陆杰,于洪巍..酶法动态动力学拆分制备R-（-）-乙酰基邻氯扁桃酸[J].化工进展,2014,(9):2425-2431,7.基金项目
国家自然科学基金（21176102、21176215、GZ935）、江苏省自然科学基金（ BK20131100）、江苏省环保科研课题基金（2012004）及浙江省自然科学基金杰出青年基金（21076181）项目。 （）
