化工进展Issue(11)：3037-3044,8.DOI:10.3969/j.issn.1000-6613.2014.11.034
新型异亮氨酸双加氧酶及其重组大肠杆菌合成羟基异亮氨酸
A novel isoleucine dioxygenase and its expression in recombinant Escherichia coli for synthesis of 4-hydroxyisoleucine
摘要
Abstract
Isoleucine dioxygenase (IDO) could hydroxylyze L-isoleucine (L-Ile) to produce 4-hydroxy-L-isoleucine (4-HIL),which increases glucose-induced insulin release and is usually used in pharmaceuticals with antidiabetic and cholesterol-reducing properties. The strain with dioxygenase activity was screened from nature via combination of reaction coloration assayed by spectrophotometer and thin-layer chromatography (TLC). The gene encoding IDO (ido) was cloned and expressed in Escherichia coli and the constructed recombinant strain exhibited the function of isoleucine hydroxylation. The reaction conditions were optimized and the yield of transformation with the recombinant whole cells was increased to more than 85% by incubating the cells overnight at 30℃. The newly identified IDO and involved recombinant whole cells would be promising to prepare 4-HIL with high efficiency.关键词
异亮氨酸双加氧酶/异亮氨酸/羟基异亮氨酸/生物转化/重组表达Key words
isoleucine dioxygenase/isoleucine/hydroxyl isoleucine/biotransformation/recombinant expression分类
生物科学引用本文复制引用
付敏杰,聂尧,穆晓清,徐岩,肖荣..新型异亮氨酸双加氧酶及其重组大肠杆菌合成羟基异亮氨酸[J].化工进展,2014,(11):3037-3044,8.基金项目
国家重点基础研究发展计划（2011CB710800）、国家高技术研究发展计划（2011AA02A209、2011AA02A210）、国家自然科学基金（21376107、21336009）、高等学校学科创新引智计划（111计划）（111-2-06）、高端外国专家项目（GDW20133200113）及江苏高校优势学科建设工程项目。 （）
