化工学报Issue(5)：1750-1758,9.DOI:10.3969/j.issn.0438-1157.2014.05.027
介孔TiO2对不同蛋白的选择性固定化性能
Mesoporous TiO2 with different selectivities of protein immobilization performance
摘要
Abstract
Mesoporous TiO2 with adjustable pore diameter, high specific surface area and excellent biocompatibility has a good application prospect in biological molecules immobilization carriers. Mesoporous TiO2-500 were prepared as the stationary phase packing in High Performance Liquid Chromatograph by calcinating H2Ti2O5 at 500℃, and the TiO2 samples were characterized by X-ray diffraction (XRD), N2 adsorption-desorption and field-emmission scanning electron microscopy (FESEM). The residence timeunder different pH of bovine serum albumin, myoglobin and lysozyme through the new chromatograph column were tested. Nearly all three kinds of proteins were immobilized at pH =4.7, and the order of residence time of three proteins was lysozyme >myoglobin > bovine serum albumin under the condition of pH=7.4 and 11. Adsorption of three kinds of proteins on the surface of the mesoporous TiO2-500 under atmospheric pressure was consistent with chromatographic results, and the order of the immobilization capacity of mesoporous TiO2-500 for three kinds of proteins was lysozyme > myoglobin >bovine serum albumin. Therefore, due to the significant difference in protein immobilization performance, the mesoporous TiO2has a potential application in protein adsorption and separation, immobilizing enzyme and other fields.关键词
介孔TiO2/蛋白质/色谱/停留时间/固定化Key words
mesoporous TiO2/protein/chromatograph/residence time/immobilization分类
化学化工引用本文复制引用
董依慧,安蓉,庄伟,姚忠,朱育丹,刘畅,陆小华..介孔TiO2对不同蛋白的选择性固定化性能[J].化工学报,2014,(5):1750-1758,9.基金项目
国家自然科学基金项目（21136004）；国家重点基础研究发展计划项目（2013CB733501）；江苏高校优势学科建设工程项目。@@@@supported by the National Natural Science Foundation of China(21136004), the National Basic Research Program of China （2013CB733501）and the Project of Priority Academic Program Development of Jiangsu High Education Institutions(PAPD) （21136004）
