化工学报Issue(1)：179-185,7.DOI:10.11949/j.issn.0438-1157.20140555
木瓜蛋白酶在离子液体双水相中的分配行为
Partitioning behavior and structural characterization of papain in ionic liquid aqueous two-phase system
摘要
Abstract
Papain was extracted by ionic liquid aqueous two-phase system. Firstly,the influence of concentration, pH, and temperature of ionic liquid on the activity of papain was investigated. Secondly, the effects of different ionic liquid aqueous two-phase systems, alkyl chain lengths and concentrations of the ionic liquid, dosage of papain, pH, temperature on the partitioning behavior of papain were discussed. [C4mim]Cl and [C4mim]Br systems were better than [C4mim]BF4 system in extraction of papain, and it was disadvantageous to extract papain at a high temperature (60℃or higher). Activity recovery of enzyme reached 95.16%and purification factor reached 1.5 under the optimum conditions:[C4mim]Cl 0.25 g·ml−1, K2HPO4 0.35 g·ml−1, pH 8.0, enzyme addition 2.0 mg·ml−1, 30℃. The result laid the experimental basis for further scale-up research and commercial production.关键词
离子液体/双水相/木瓜蛋白酶/酶活性回收率/纯化因子/萃取/蛋白质Key words
ionic liquid/aqueous two-phase system/papain/activity recovery of enzyme/purification factor/extraction/protein分类
轻工纺织引用本文复制引用
王伟涛,蒋志国,张海德,彭健,许英豪,董安华,杨雪芳,蒋欣欣..木瓜蛋白酶在离子液体双水相中的分配行为[J].化工学报,2015,(1):179-185,7.基金项目
国家自然科学基金项目（31260401）。@@@@supported by the National Natural Science Foundation of China (31260401) （31260401）
