化工学报Issue(7)：2613-2619,7.DOI:10.11949/j.issn.0438-1157.20141929
大肠杆菌温敏耐热系统的构建与应用
Construction and application of temperature sensitive thermotolerant system in Escherichia coli
摘要
Abstract
The heat stress produced in fermentation process can not only affect the growth and reproduction of microorganisms, but also increases production cost from cooling and temperature control. The improvement of strain thermotolerance is highly desire for fermentation industry, which could significantly go up productivity and reduce production cost. So in this study, an artificial design and synthesis RNA switch with temperature sensitivity is employed to regulate and control the heat shock protein Dnak from Thermoanaerobacter tengcongensis MB4, which leads to the thermotolerance improvement of Escherichia coli. This improvement not only alleviated the burden of overexpressing heat shock protein in Escherichia coli at 37℃, but also enhanced its productivity at high temperature. The results of application test show that at 40℃ the output of lysine produced by E. coli increases by 2.95 folds, when compared with control group. This success could be twilight for the development of new method to prepare thermotalerance microorganisms.关键词
生物技术/合成生物学/发酵/耐热性/RNA温敏开关/热激蛋白/赖氨酸Key words
biotechnology/synthetic biology/fermentation/thermotolerance/temperature sensitive RNA switch/heat shock protein/lysine分类
生物科学引用本文复制引用
贾海洋,孙欢,孙翔英,冯旭东,李春..大肠杆菌温敏耐热系统的构建与应用[J].化工学报,2015,(7):2613-2619,7.基金项目
国家重点基础研究发展计划项目（2011CBA00800）；国家自然科学基金项目（21376028）；国家杰出青年科学基金项目（21425624）。 （）
