环境保护科学Issue(6)：83-87,130,6.
土壤中胡敏酸提取方法的优化
Optimization of Humic Acid Extraction in Soils
摘要
Abstract
On the basis of humic acid extraction method recommended by the International Humic Substances Society (IHSS), the soil prepared by mixture of organic soil and humic acid is taken as the study target and ultrasound is used as the auxiliary condition for humic acid extraction. Humic acid extraction method is optimized through batch experiments in the paper. The results show that on the basis of recovery rate and precision of humic acid extraction, the optimized extraction method at room temperature is as follows: liquid-to-soil ratio is 8:1; extraction is for 3 times; concentration of NaOH solution is 0.05mol/L; ultrasonic power is 120W; and ultrasonic time for extraction is 30 minutes. Under such optimized conditions, the recovery rate of humic acid from soils is 94.73% 1.50%, significantly more than that of the method recommended by IHSS (64.76% 0.28%). The coefficient of variation (CV) is 1.59%, less than 10%. Compared with the method recommended by IHSS, the optimized extraction method is much more advantageous with such features as high extraction recovery rate of humic acid, resource saving, less denaturation of humic acid and shorter extraction time.关键词
胡敏酸/超声/提取方法/优化Key words
Humic Acid/Ultrasound/Extraction Method/Optimization分类
农业科技引用本文复制引用
谯华,李恒,周从直,郝全龙,杜蓉,史永刚,毛华军..土壤中胡敏酸提取方法的优化[J].环境保护科学,2014,(6):83-87,130,6.基金项目
总后勤部项目（CY112C017）；解放军后勤工程学院学术创新科研项目（YZ11-43301）；浙江省固体废物处理与资源化重点实验室开放课题重点实验室开放项目（SWTR-2012-04）资助 （）
