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大庆龙凤湿地底泥氨氧化细菌与氨氧化古菌多样性分析

马茹霞 晏磊 王国兴 王长虹 贾军 邓兵 沈婷婷 王伟东

黑龙江八一农垦大学学报Issue(2):69-73,78,6.
黑龙江八一农垦大学学报Issue(2):69-73,78,6.DOI:10.3969/j.issn.1002-2090.2015.02.016

大庆龙凤湿地底泥氨氧化细菌与氨氧化古菌多样性分析

Diversity of Ammonia-oxidizing Bacteria and Ammonia-oxidizing Archaea in Sediment of Longfeng Wetland in Daqing

马茹霞 1晏磊 1王国兴 1王长虹 1贾军 1邓兵 1沈婷婷 2王伟东1

作者信息

  • 1. 黑龙江八一农垦大学生命科学技术学院,大庆 163319
  • 2. 上海岩土工程勘察设计研究院有限公司
  • 折叠

摘要

Abstract

To study the diversity of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in sediment of Longfeng wetland in Daqing,amoA clone libraries of AOB and AOA were constructed. The libraries were analyzed by PCR-based restriction fragment length polymer-phism(RFLP)technique. The results showed that 3 OTUs of AOB and AOA were obtainted from Longfeng wetland sediment,respectively. All sequences of AOB belonged to Nitrosomonas,and all sequences of AOA belonged to Crenarchaeota,and it had the high sequence homology with the sequences that were obtained from estuary sediments.

关键词

湿地底泥/氨氧化细菌/氨氧化古菌/多样性

Key words

wetland sediment/ammonia-oxidizing bacteria(AOB)/ammonia-oxidizing archaea(AOA)/diversity

分类

资源环境

引用本文复制引用

马茹霞,晏磊,王国兴,王长虹,贾军,邓兵,沈婷婷,王伟东..大庆龙凤湿地底泥氨氧化细菌与氨氧化古菌多样性分析[J].黑龙江八一农垦大学学报,2015,(2):69-73,78,6.

基金项目

国家自然科学基金(31270536);黑龙江省高校科技创新团队项目(2012TD006)。 ()

黑龙江八一农垦大学学报

1002-2090

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