黑龙江八一农垦大学学报Issue(3)：57-65,87,10.DOI:10.3969/j.issn.1002-2090.2015.03.014
小分子团磁化水对芸豆清蛋白浸出效果的影响
Effects of Magnetized Water with Small Molecules Mass on Albumin Extraction from Kidney Bean
摘要
Abstract
Using Kenyun 2 kidney beans as raw material and tap water and pure water as comparison tests,the infusion effects of magnetized water with small molecules mass to kidney bean were studied,and the extraction parameters of albumin from kidney bean were optimized. Through single factor and response surface analysis of four factors such as extraction temperature,extraction time, ratio of liquid to material and particle size,the results showed that the optimum extraction conditions was as follows:extraction temperature 31 ℃,extraction time 2 h,ratio of liquid to material 22∶1,particle size 60 mesh. Under such conditions,the extraction yield of magnetized water with small molecules group was 48.96% and higher than that of tap water group (42.79%)and purified water group (40.87%).SDS-PAGE result showed that the molecular weight of the extracted protein was about 42.01 kDa,and the protein was albumin.关键词
芸豆清蛋白/小分子团磁化水/提取/SDS-PAGEKey words
kidney bean albumin/magnetized water with small molecules mass/extraction yield/SDS-PAGE分类
轻工纺织引用本文复制引用
包国凤,刁静静,井雪莲,张丽萍..小分子团磁化水对芸豆清蛋白浸出效果的影响[J].黑龙江八一农垦大学学报,2015,(3):57-65,87,10.基金项目
国家杂粮工程技术研究中心组建项目（2011FU125X07）。 （）
