摘要
Abstract
In order to optimize the SSR molecular marker system and rich the diversity of germplasm resources , taking maize inbred line CK and its seven mutants as materials ,the experiment system was optimized and the genetic difference between mutants and the corresponding basic material was studied based on SSR markers with selected 23 pairs of polymorphism SSR primers .The results showed that(1)The optimization of SSR mo-lecular marker experiment system could effectively improve the efficiency of the experiment ,reduce the experi-mental error ;(2)By using the 23 pairs of SSR primer ,a total of 59 polymorphic amplified fragments was pro-duced .The average number of allele per SSR locus was 2 .565 .The polymorphism information content(PIC)for the SSR loci varied from 0 .110 to 0 .525 with an average of 0 .331 .The amplitude of genetic diversity indexes ranged from 0 .234 to 1 .061 with an average of 0 .643 .The phenomenon indicated that massive genetic variation could appear in maize(CK)progenies due to mutagenesis .关键词
SSR/体系优化/遗传多样性Key words
SSR markers/system optimization/genetic variation分类
农业科技
