黑龙江农业科学Issue(1)：6-9,4.DOI:10.11942/j.issn1002-2767.2015.01.0006
二倍体马铃薯原生质体培养与植株再生
Culture and Plantlets Regeneration from Protoplast of Diploid Potato
摘要
Abstract
Protoplast culture is the foundation of somatic hybridization and an important method to acquire mu‐tant material .Taking four introduced diploid potato as materials ,the effect of osmotic concentration ,enzymoly‐sis conduction ,enzymolysis time and pretreatment on yield and vitality of protoplast were studied .The results indicated that pretreatment was a key factor for surviving ,division and development of protoplast .The proto‐plast yield and vitality of treatment D were the best .The osmotic concentration suitable for protoplast was 0 .35~0 .40 m・L‐1 .The suitable enzymolysis time for RH2 and RH3 was 4 h ,RH1 and RH4 was 4 .5 h .Enzy‐matic hydrolysate was 2 .0% Cellulase RS Onozuka and 0 .2% Pectolyase Y‐23 .The protoplast culture was greatly influenced by genotypes .Regeneration plantlets of two diploid potato materials were successfully gained .关键词
二倍体马铃薯/原生质体/再生植株Key words
diploid potato/protoplast/regeneration plantlets分类
农业科技引用本文复制引用
刘文萍,刘建新,南相日,张丽娟,盛万民,张举梅..二倍体马铃薯原生质体培养与植株再生[J].黑龙江农业科学,2015,(1):6-9,4.基金项目
科技部国家国际科技合作资助项目 （）
