黑龙江农业科学Issue(1)：50-56,7.DOI:10.11942/j.issn1002-2767.2015.01.0050
东北地区土壤中高毒力虫生真菌菌株的筛选
Screening of High Virulent Strains of Entomogenous Fungi in Soil of Northeast China
摘要
Abstract
In order to screen high virulence fungus strains ,using baiting soil samples with larvae of Galleria mellonella ,thirty‐six soil samples were collected from different ecotype areas in Northeast China .Each soil sample was baited with 30 larvae of third or fourth instar ,fourteen days later ,a total of 454 larvae which had been infected .The occurrence frequency of entomopathogenic fungi in high density area was 80% in Tengjia‐weizi of Kedong county .There were 301 fungus isolated from the cadavers ,most isolates were Beauveriabassi‐ana ,a little Metarrhizium anisopliae and others ,there were 41 fungus isolated ,they were tested under labora‐tory conditions against Myzus persicae and Plutella xylostella .Each isolate was bioassayed under the concen‐trated standard spray of 1 .0 × 107 conidia・mL‐1 .Isolates of JSJ‐10、QG1‐1、MBD‐2、BEE‐15、HYD‐11、MLX‐1 and FN‐9 were more virulent to Myzus persicae ,causing a corrected mortality of 96 .6% 、96 .6% 、96 .1% 、93 .2% 、92 .8% 、87 .6% and 82 .8% after spraying 7 d .As for Plutella xylostella ,isolates of WMS‐12 and MBD‐1 which were Metarrhizium anisopliae were more virulent ,causing a corrected mortality each of 83 .3%and 69 .3% af ter dipping 5 d .关键词
大蜡螟/虫生真菌/桃蚜/小菜蛾/生物测定/白僵菌/绿僵菌Key words
Galleria mellonella/entomogenous fungi/Myzus persicae/Plutella xylostella/bioassay/Beau-veriabassiana/Metarrhizium anisopliae分类
农业科技引用本文复制引用
王爽,李新民,刘春来,夏吉星,杨帆,王克勤,刘兴龙..东北地区土壤中高毒力虫生真菌菌株的筛选[J].黑龙江农业科学,2015,(1):50-56,7.基金项目
黑龙江省农业科技创新工程资助项目 （）
