黑龙江农业科学Issue(1)：112-115,4.DOI:10.11942/j.issn1002-2767.2015.01.0112
密楝果实中绿原酸含量的测定
Determination of Chlorogenic Acid in Evodia lenticellata Huang
摘要
Abstract
In order to further develop and utilize Evodia lenticellata Huang resources ,the chlorogenic acid were extracted from E .lenticellata Huang using 50% methanol ,and the conditions of the ultrasonic wave as follows:extraction ultrasonic power of 240 W ,ultrasonic frequency of 40 kHz ,temperature of 60℃ and ultrasonic treat‐ment time of 30 min .Samples were analyzed on an Inertsil ODS‐3C18 column(4 .6 × 150 mm ,5μm) using aceto‐nitrile‐0 .4% phosphoric acid(8∶92)as mobile phase ,speed of flow was 1 .0 mL・min‐1 ,the examination wave length was 327 nm and the column temperature was 30℃ .The chlorogenic acid showed a good linearity in the range of 20 .8~104 μg・mL‐1 ,egression equation was Y=32 304X -44 968(R2 =0 .999 9)and the average re‐covery was 99 .59% (RSD=1 .69% ,n=6) .The chlorogenic acid is richer in E .lenticellata Huang and there are some different in the content of chlorogenic acid in E .lenticellata Huang among different origins .The estab‐lished method is simple ,credible ,accurate ,repeatable and culd be used for the quality control of E .lenticellata H uang .关键词
密楝果实/绿原酸/含量测定Key words
Evodia lenticellata Huang/chlorogenic acid/content determination分类
农业科技引用本文复制引用
王星,张娜,赵桦..密楝果实中绿原酸含量的测定[J].黑龙江农业科学,2015,(1):112-115,4.基金项目
陕西省教育厅专项科研计划资助项目（12JK0842）；陕西理工学院研究生创新基金资助项目 （）
