黑龙江农业科学Issue(2)：147-150,151,5.DOI:10.11942/j.issn1002-2767.2015.02.0147
大片段DNA克隆载体的研究进展
Research Progress of Large Fragment DNA Cloning Vector
摘要
Abstract
DNA cloning techniques is a very important technology in molecular biology and genetic engineering research .Since the first pSC101 plasmid cloning vector as the first cloning vector ,the rapid development of mo‐lecular biology techniques ,the overall structure of the cloning vector ,the capacity‐load capacity and conversion efficiency had been greatly improved .Through the reviews of the development situation of the cloning vector ,as well as construction and application of large fragments of DNA libraries ,and large pieces of DNA genetic trans‐formation of development were put forward .关键词
克隆载体/BIBAC/大片段DNAKey words
cloning vector/BIBAC/large fragment DNA分类
生物科学引用本文复制引用
于洋,蒋世翠,王康宇,薛菲,张美萍,王义..大片段DNA克隆载体的研究进展[J].黑龙江农业科学,2015,(2):147-150,151,5.基金项目
国家高技术研究发展计划（863计划）资助项目 （）
