湖南大学学报（自然科学版）Issue(6)：96-100,5.
响应面法优化 S2菌株的培养条件∗
Optimization of Culture Conditions of S2 Strain by Response Surface Method
摘要
Abstract
S2 strain that highly produces biosurfactant was screened from the oilfield water of three plants in Daqing Oilfield in the rapid screening method,and the S2 strain was identified as Bacillus sp..Through FT-IR and ion identification test,S2 biosurfactant was identified as nonionic lipopeptide biosurfactant.This experiment treated emul-sification index (E24 )of S2 strain fermentation broth as the object.Through response surface optimization method, the culture conditions (inoculum,pH,temperature and rotation speed)of the S2 strain were optimized.On the basis of response surface model,the optimum pH value was 7.2 and the temperature was 43.5 ℃,inoculation of 5.2% (V/V),rotation speed was 162 r/min through the quadratic regression equation.Under the optimal condition,the best E24 of S2 strain fermentation broth was 81.20%.关键词
发酵液/红外光谱法/响应面实验/乳化/菌株Key words
fermentation broth/infrared spectral measurements/response surface experiments/emul-sification/strain分类
能源科技引用本文复制引用
刘江红,任静薇,贾云鹏,杨林峰,于博,李航..响应面法优化 S2菌株的培养条件∗[J].湖南大学学报（自然科学版）,2015,(6):96-100,5.基金项目
黑龙江省教育厅科技公关项目(12531064) （12531064）
