湖南大学学报（自然科学版）Issue(6)：107-112,6.
湘江四环素及抗性基因含量特征及其季节变化∗
Occurrence and Seasonal Changes of Tetracycline Antibiotics and Antibiotic Resistance Genes in the Xiang River
摘要
Abstract
This paper adopted high-performance liquid chromatography-mass spectrograph (HPLC-MS)to investigate the occurrence and seasonal changes of tetracycline antibiotics in the Xiang River and its sediments.Five representative antibiotic resistance genes (tet A,tet C,tet G,tet M,tet X)in water and sediment samples were quantified with real-time PCR.The concentrations of tetracycline showed sensitive seasonal changes,ranging from 1 6.23 ng·L-1 to 496.73 ng·L-1 in the water samples,and from 347.77 ng·kg-1 to 3 829.75 n·kg-1 in the sediment samples,respectively.The target resistance genes were de-tected in all samples.Compared with other resistance genes,tet A and tet C were higher in absolute copy number and relative abundance,suggesting that the tet A and tet C were dominant in the Xiang River.Sta-tistical analysis indicated a positive correlation between the relative abundance of tet A and tet C in the wa-ter samples and the sediment samples in winter.There was no linearly relationship between antibiotics and resistance genes.关键词
基因/湘江/四环素抗生素Key words
genes/the Xiang River/tetracycline分类
资源环境引用本文复制引用
范长征,王聪,鲁伦慧,刘谦,曾光明..湘江四环素及抗性基因含量特征及其季节变化∗[J].湖南大学学报（自然科学版）,2015,(6):107-112,6.基金项目
国家自然科学基金资助项目(50908079),National Natural Science Foundation of China(50908079) （50908079）
