华南农业大学学报Issue(4)：22-25,4.
20味中药提取物对无乳链球菌的体外抑菌活性研究
Effects of twenty traditional Chinese medicine extracts against Streptococcus agalactiae in vitro
摘要
Abstract
Objective] Screening for the traditional Chinese medicines which have good antibacterial effects on Streptococcus agalactiae in vitro.[Method] Using ethanol reflux and ultrasonic method , ex-tracts of 20 different traditional Chinese medicines were obtained .Minimal inhibitory concentration ( MIC) and minimal bactericidal concentration ( MBC) of Coptis chinensis and other 19 extracts of tradi-tional Chinese medicines on St.agalactiae were detected by macrodilution broth method , and combined antibacterial effects in vitro of them were also observed .[Result and conclusion] The MICs of the ex-tracts obtained from Prunus mume, Coptis chinensis, Scutellaria baicalensis , Polygonum cuspidatum and Sanguisorba officinalis against St.agalactiae ranged from 7.80 to 31.25 mg/mL.The MICs of the ex-tracts obtained from Taraxacum mongolicum, Artemisia scoparia, Fraxinus rhynchophylla, Artemisia arg-yi, Phellodendron chinense, Houttuynia cordata, Violae yedoensis and Pulsatilla chinensis ranged from 62.50 to 125.00 mg/mL.The MICs of the extracts obtained from Po.multiflorum, Sophora flavescens, Andrographis paniculata, Eucommia ulmoides, Forsythia suspensa, Lonicera japonica and Prunella vul-garis ranged from 250.00 to 500.00 mg/mL.The results of joint bacteriostatic test showed that the frac-tional inhibitory concentration index ( FICI) of the extracts obtained from Sc.baicalensis, Po.cuspida-tum, Sa.officinalis and C.chinensis was less than or equal to 1.The FICI of Po.cuspidatum, Sc.ba-icalensis and Sa.officinalis was more than 2.C.chinensis, Sc.baicalensis, Po.cuspidatum and Sa.offi-cinalis showed a good antibacterial effect against St.agalactiae in vitro.The combination of Sa.officina-lis and C.chinensis was characterized by a additive effect , whereas the combination of C.chinensis, Sc. baicalensis and Po.cuspidatum was characterized by a synergism effect .The combination of Sa.officina-lis and Sc.baicalensis, Po.cuspidatum showed antagonism effect .关键词
无乳链球菌/中药提取物/抗菌活性Key words
Streptococcus agalactiae/traditional Chinese medicine extract/antimicrobial activity分类
农业科技引用本文复制引用
彭练慈,殷中琼,贾仁勇,李莉,代如意,曲径,刘明辉,陈萍..20味中药提取物对无乳链球菌的体外抑菌活性研究[J].华南农业大学学报,2014,(4):22-25,4.基金项目
四川省青年科技创新研究团队（2013TD0015）；“十二五”农村领域国家科技计划项目 （）
