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1个新的玉米半透明皱缩胚乳突变体的遗传分析及基因定位

崔喜艳 胡广宇 孙小杰 佟珊珊 陈众峰 刘相国

华南农业大学学报Issue(5):31-35,56,6.
华南农业大学学报Issue(5):31-35,56,6.DOI:10.7671/j.issn.1001-411X.2014.05.006

1个新的玉米半透明皱缩胚乳突变体的遗传分析及基因定位

A genetic analysis and gene mapping of a new translucent and shrunken endosperm mutant in maize

崔喜艳 1胡广宇 2孙小杰 1佟珊珊 3陈众峰 1刘相国1

作者信息

  • 1. 吉林农业大学生命科学学院,吉林长春130118
  • 2. 吉林省农业科学院农业生物技术研究所,吉林长春130124
  • 3. 吉林省中玉农业有限公司,吉林长春130012
  • 折叠

摘要

Abstract

Objective To analyze the formation mechanism of translucent and shrunken maize endo-sperm.[Method] The mutant M-E-479 of homozygous maize was used as experimental materials in our study .Through phenotypic identification , genetic analysis , main ingredients measurement and scanning electron microscopy , mapping population of M-E-479 was selected and map-based cloning technology was performed to locate the mutant gene .[Result and conclusion] The result showed that the amount of fat in-creased , while the protein and starch decreased , and the starch grains changed in size , shape and space configuration in the mutant endosperm of M-E-479 .The mutant gene is located between SSR markers bn-lg1074 and umc1506 of chromosome 10 by using F2 population .Meanwhile , the genetic distance is about 3.6 cM and the physical distance is about 2.2 Mb.The results suggest that M-E-479 is a new translucent and shrunken maize endosperm mutant , and its mutant character may be controlled by a recessive single gene .

关键词

玉米/胚乳突变体/遗传分析/基因定位

Key words

maize/endosperm mutant/genetic analysis/gene mapping

分类

农业科技

引用本文复制引用

崔喜艳,胡广宇,孙小杰,佟珊珊,陈众峰,刘相国..1个新的玉米半透明皱缩胚乳突变体的遗传分析及基因定位[J].华南农业大学学报,2014,(5):31-35,56,6.

基金项目

国家自然科学基金(31170731,31200611);上海市科学技术委员会资助项目(10DZ2271800);吉林省科技发展计划项目 ()

华南农业大学学报

OA北大核心CSCDCSTPCD

1001-411X

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