华南农业大学学报Issue(5):31-35,56,6.DOI:10.7671/j.issn.1001-411X.2014.05.006
1个新的玉米半透明皱缩胚乳突变体的遗传分析及基因定位
A genetic analysis and gene mapping of a new translucent and shrunken endosperm mutant in maize
摘要
Abstract
Objective To analyze the formation mechanism of translucent and shrunken maize endo-sperm.[Method] The mutant M-E-479 of homozygous maize was used as experimental materials in our study .Through phenotypic identification , genetic analysis , main ingredients measurement and scanning electron microscopy , mapping population of M-E-479 was selected and map-based cloning technology was performed to locate the mutant gene .[Result and conclusion] The result showed that the amount of fat in-creased , while the protein and starch decreased , and the starch grains changed in size , shape and space configuration in the mutant endosperm of M-E-479 .The mutant gene is located between SSR markers bn-lg1074 and umc1506 of chromosome 10 by using F2 population .Meanwhile , the genetic distance is about 3.6 cM and the physical distance is about 2.2 Mb.The results suggest that M-E-479 is a new translucent and shrunken maize endosperm mutant , and its mutant character may be controlled by a recessive single gene .关键词
玉米/胚乳突变体/遗传分析/基因定位Key words
maize/endosperm mutant/genetic analysis/gene mapping分类
农业科技引用本文复制引用
崔喜艳,胡广宇,孙小杰,佟珊珊,陈众峰,刘相国..1个新的玉米半透明皱缩胚乳突变体的遗传分析及基因定位[J].华南农业大学学报,2014,(5):31-35,56,6.基金项目
国家自然科学基金(31170731,31200611);上海市科学技术委员会资助项目(10DZ2271800);吉林省科技发展计划项目 ()