华南农业大学学报Issue(6)：83-88,6.
25个油茶优良无性系的遗传分析与分子鉴别
Genetic analyses and molecular identification of 25 superior clones of Camellia oleifera
摘要
Abstract
[Objective]This study aimed to lay a theoretical foundation for rational use of Camellia oleifera germplasm resource and provide technical support for the conservation of elite C.oleifera varieties.[Method]SRAP molecular markers were used to assess the genetic diversity and molecular identification of 25 superior clones of C.oleifera from Jiangxi Province .[Result and conclusion]Three hundred ard forty seven sites were amplified by 11 primer combinations of SRAP , among which 332 were polymorphic sites . The percentage of polymorphic sites was 95.68%.The result of cluster analysis indicated that the genetic distance of the 25 superior clones ranged from 0.255 6 to 0.738 4, and the average genetic distance was 0.599 9 , indicating that these cultivars had similar geographical origins .The 25 superior clones were di-vided into five groups by cluster analysis according to genetic distance 0.528 0 .DNA fingerprints of the 25 superior clones were constructed using the screened primer combinations .The fingerprint constructed by each primer combination can be used for molecular identification of the 25 superior clones .关键词
油茶/优良无性系/SRAP/遗传分析/分子鉴别Key words
Camellia oleifera/superior clone/SRAP/genetic analysis/molecular identification分类
农业科技引用本文复制引用
孙佩光,奚如春,李俊成,欧阳昆唏,钟燕梅,陈晓阳..25个油茶优良无性系的遗传分析与分子鉴别[J].华南农业大学学报,2014,(6):83-88,6.基金项目
广东省自然科学基金团队项目（9351064201000002）；广东省林业科技创新专项 （）
