华南农业大学学报Issue(2)：117-120,4.
聚丙烯酰胺电泳条带中微量 DNA 片段的回收与再扩增方法的改进
Improved methods to recover and reamplify trace DNA fragments from the polymorphic PAGE band
摘要
Abstract
Objective]To optimize the methods of recovery and reamplification of trace DNA fragments from polymorphic polyacrylamide gel electrophoresis (PAGE) bands.[Method] Effects of four methods of dissolving polyacrylamide gel and recovery efficiencies of trace nucleic acids were obtained .[Result and conclusion] Dissolving/Bing buffer from Bioteke kit showed high recovery rates of DNA fragments from PAGE gels with only 1 .0-7 .5 ng nucleotide .Effects of amplification positively correlated with re-covery efficiencies .The separation of liquid mixture and impurities was the key to successful amplifica-tion.This research forms a fast , efficient DNA recycling and amplification method from polymorphism PAGE, which provides a new approach to analyze the causes of genetic diversity .关键词
聚丙烯酰胺凝胶电泳/微量DNA带回收/DNA片段再扩增Key words
PAGE/trace DNA fragments recovery/reamplification of DNA sequence分类
生物科学引用本文复制引用
许美容,贾奥琳,郑正,邓晓玲..聚丙烯酰胺电泳条带中微量 DNA 片段的回收与再扩增方法的改进[J].华南农业大学学报,2015,(2):117-120,4.基金项目
国家自然科学基金（ G31201480）；农业部公益性行业（农业）专项（201003067）；广东省自然科学基金 （）
