河南农业科学Issue(6)：25-29,5.
普通野生稻胚性愈伤组织诱导体系的建立
Study on Differentiation and Dedifferentiation of Oryza ruf ipogon Griff
摘要
Abstract
The N6D and NB medium containing different hormones (IAA ,NAA ,2 ,4 D ,6 BA and KT)were used to study the callus rate of Oryza rufipogon Griff ,so as to determine the best medium for O .ruf ipogon Griff .And the shoot induction rate from embryonic calli was also ob-served .The result showed that ,after the seeds were placed on medium for 7d ,the callus ratio from N6D medium with 6.0 mg/L 2 ,4 D ,3.0 mg/L NAA ,3.0 mg/L IAA ,1.5 mg/L KT was 56.7% ,while the diameter of callus reached to 0.31 cm .And then the callus of O .ruf ipogon Griff were transferred to MS dedifferentiation medium for 24 d ,the callus became the green plants .This is a quick system for differentiation and dedifferentiation of O .ruf ipogon Griff .关键词
普通野生稻/愈伤组织/脱分化/分化Key words
Oryza rufipogon Griff/callus/differentiation/dedifferentiation分类
农业科技引用本文复制引用
潘英华,梁云涛,陈成斌,梁世春,段俊枝,余建平,黄娟,徐志健..普通野生稻胚性愈伤组织诱导体系的建立[J].河南农业科学,2014,(6):25-29,5.基金项目
广西科学研究与技术开发计划项目（桂科攻11230013 A ）；广西自然科学基金项目（桂科青0991053，桂科青0933001z ，桂科青0832001z）；广西农业科学院基本科研业务专项（200923）；广西农业科学院面上项目（2007027）；广西农业科学院院基金项目（200901Z ）；广西农业科学院发展基金项目 （）
