猪伪狂犬病毒野毒株 SYBR Green Ⅰ实时荧光定量 PCR 诊断试剂盒的研制OA北大核心CSCDCSTPCD
Development of SYBR Green Ⅰ Real-time Quantitative PCR Kit for Detection of PRV Wild-type Strains
根据GenBank中猪伪狂犬病毒（PRV ）gE基因序列设计特异性的引物，PCR扩增获得PRV gE基因片段，构建重组质粒pMD18 T gE ，作为阳性标准品。对SYBR Green Ⅰ实时荧光定量PCR反应条件进行优化，建立猪PRV SYBR Green Ⅰ实时荧光定量PCR诊断方法，研制诊断试剂盒。扩增产物的熔解曲线分析结果显示只出现单特异峰，无引物二聚体，对猪圆环病毒（PC V 2）、猪细小病毒（PPV ）、PRV （gE基因缺失株）、猪…查看全部>>
According to the gene sequences of gE gene of PRV in GenBank ,one pairs of specific primer was designed for amplifying the specific fragments of gE gene .Then gE gene of PRV amplified by PCR was cloned into pMD18-T vector and it was used as positive standard .After optimization of annealing temperature and primers concentrations ,a SYBR GreenⅠ real-time quantitative PCR kit was developed for detection of PRV wild-type strains .The melting curve analysis usin…查看全部>>
余波;周思旋;谭诗文;徐景峨;史开志;杨莉
贵州省畜牧兽医研究所，贵州贵阳550005贵州省畜牧兽医研究所，贵州贵阳550005贵州省畜牧兽医研究所，贵州贵阳550005贵州省畜牧兽医研究所，贵州贵阳550005贵州省畜牧兽医研究所，贵州贵阳550005贵州省畜牧兽医研究所，贵州贵阳550005
农业科技
猪伪狂犬病毒野毒株gE基因荧光定量PCR诊断试剂盒
PRVwild-type strainsgEreal-time quantitative PCRdiagnostic kit
《河南农业科学》 2014 (6)
128-131,144,5
贵州省科技厅农业攻关项目（［2010］3085）；贵州省畜禽健康养殖技术创新能力建设项目（［2010］4004）
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