河南农业科学Issue(6)：149-151,3.
日本鹌鹑艾美耳球虫单卵囊分离及雏鹑扩增研究
Eimeria uz ura Single Oocyst Isolation and Amplification in Young Quails
摘要
Abstract
To obtain a large number of pure-bred E .uz ura ,quails’ coccidia oocysts were collected using the saturated brine flotation method and cultured at 26 ℃ .According to morphological identification , single sporulated oocyst of E .uzurawas separated and amplified in young quails .The results showed that sporulation of oocysts occurred for 14 h at 26 ℃ .seven-day-old artificially-reared coccidian-free quails were inoculated orally with 1 and 200 sporulated oocysts of E .uz ura ,the prepatent period was 6 d and 5 d ,and discharge of oocysts continued for 8-10 d and 11 d ,respectively .关键词
日本鹌鹑艾美耳球虫/单卵囊分离/卵囊孢子化/扩增Key words
Eimeria uz ura/single oocyst isolation/oocysts sporulation/amplification分类
农业科技引用本文复制引用
张敏,韩琼琼,李清州,王芳,梁楠,张龙现,菅复春,宁长申..日本鹌鹑艾美耳球虫单卵囊分离及雏鹑扩增研究[J].河南农业科学,2014,(6):149-151,3.基金项目
国家现代农业产业技术体系建设专项 （）
