河南农业科学Issue(9)：18-23,6.
水稻OsLEA5c基因的克隆、表达和抗逆性分析
Cloning,Expression and Resistance Analysis of OsLEA5c in Rice(Oryza sativa L.)
摘要
Abstract
To study the resistance of the late embryogenesis abundant protein gene OsLEA5c,the cDNA sequence of OsLEA5c from rice was cloned by RT-PCR.Sequence analysis showed that OsLEA5c consisted of 456 bp open reading frame(ORF)that encoded 151 amino acids with a calculated molecular mass of 16.55 kD and a theoretical pI of 5.07.The deduced OsLEA5c protein was a strongly hydrophobic protein with the grand average of hydropathy(GRAVY)value of 0.020 and had a Pfam:LEA_2 domain architecture at position 44-140.The OsLEA5c protein shared 45.14%-61.59% identity with other members of subgroup 5C LEA proteins from plants.The phylogenetic relationship between related group 5 LEA proteins from different plants was analyzed,indicating that OsLEA5c belonged to subgroup 5C.Recombinant expression plasmid pET32a-OsLEA5c was constructed,and then was transformed into E.coli BL21(DE3)pLysS to obtain the recombinant strains.A fusion protein with a molecular weight of about 34 kD was expressed in the recombinant strains.The results of stress tolerance assay demonstrated that the recombinant strains producing OsLEA5 c fusion protein improved the resistance against diverse abiotic stresses,such as heat,high salinity,hyperosmosis and freeze-thaw.The findings illustrated that OsLEA5c plays an important role in resistance against diverse abiotic stresses during the maturation and desiccation phases of rice seed development.关键词
水稻/胚胎发育后期丰富蛋白/OsLEA5c基因/表达/抗逆性Key words
Oryza sativa L./late embryogenesis abundant protein/OsLEA5c gene/expression/resistance分类
农业科技引用本文复制引用
胡廷章,杨俊年,周大祥,黄小云,陈再刚..水稻OsLEA5c基因的克隆、表达和抗逆性分析[J].河南农业科学,2014,(9):18-23,6.基金项目
重庆市自然科学基金项目（CSTC，2012jjA80009）；重庆市教委科学技术研究项目 （）
