河南农业科学Issue(10)：127-130,4.
海产品中霍乱弧菌实时浊度LAMP 检测方法的建立
Establishment of A Real-time Turbidimeter-based Loop-mediated Isothermal Amplification for Detection of Vibrio cholera in Seafood
摘要
Abstract
In order to establish a rapid,sensitive,specific,and effective method for diagnosis of vibrio cholera,to prevent and control the bacteria infection,a loop-mediated isothermal amplification(LAMP)assay was developed with a set of primers designed for V.cholera ctxA gene.The real-time monitoring of the LAMP reaction was achieved by real-time turbidimeter,and the specificity and sensitivity were tested.The results showed that V.cholera with ctxA gene exhibited typical curves,and 7 strains of other bacteria concurred withV.cholera in seafood.The detection limitation of this method was 81 fg/μL for purified genomic DNA.Furthermore,a total of 150 seafood samples were tested by the established method and the virulence ctxA gene was detected from 2 samples,which was accordant with the result by the real-time quantitative PCR. This assay is rapid and reliable for detection ofV.cholera.关键词
霍乱弧菌/环介导等温扩增/实时浊度/ctxA 基因/检测方法Key words
Vibrio cholera/loop-mediated isothermal amplification/real-time turbidimeter/ctxA gene/detection method分类
农业科技引用本文复制引用
胡兴娟,沈飚,江玲丽,王莲芳,滕跃,朱竹君..海产品中霍乱弧菌实时浊度LAMP 检测方法的建立[J].河南农业科学,2014,(10):127-130,4.基金项目
浙江检验检疫局科技计划项目 （）
