河南农业科学Issue(10)：131-134,4.
“桑蚕乳酸脱氢酶基因的克隆与表达分析
Cloning and Expression Profiling Analysis of Bombyx mandarina LDH Gene
摘要
Abstract
In order to explore the functions of lactate dehydrogenase(LDH),using the different tissues of 3 day of fifth instar larvae and the different developmental stages individuals,the LDH gene from Bombyx mandarina was cloned by polymerase chain reaction.The results showed that the open reading frame of LDH was 996 bp,encoding 331 amino acid,containing 1 exon,no intron,and a predicted pI of 6.76 and molecular weight of 36·35 kD.The deduced amino acid sequence was highly conserved.The predicted analysis showed that the LDH was located in the cytoplasm.Transcriptal expression pattern revealed that the LDH was expressed in the tissues (malpig-hian tubule,midgut,epidermis,fat body,silk gland,blood,testis,ovary ) and developmental stages(egg,larva,pupa,moth),and the expression level was almost consistent. LDH may play an important physiological function in vital movement of Bombyx mandarina .关键词
野桑蚕/乳酸脱氢酶基因/克隆/表达Key words
Bombyx mandarina/LDH gene/cloning/expression分类
农业科技引用本文复制引用
武安泉,张永亮,赵锦慧..“桑蚕乳酸脱氢酶基因的克隆与表达分析[J].河南农业科学,2014,(10):131-134,4.基金项目
国家自然科学基金项目（31271627）；河南省教育厅自然科学基金项目（2010A230016）；周口师范学院博士科研启动基金项目；周口师范学院校级重点学科建设经费资助项目 （）
