河南农业科学Issue(3)：148-151,4.DOI:10.15933/j.cnki.1004-3268.2015.03.033
猪博卡病毒NP1基因的克隆及其生物信息学分析
Cloning and Bioinformatics Analysis of Porcine Bocavirus NP1 Gene
郑英帅 1左奕 1柴瑞影 1戚妍 1李雅楠 1袁万哲 1孙继国1
作者信息
- 1. 河北农业大学 动物医学院，河北 保定071001
- 折叠
摘要
Abstract
A pair of primers were designed based on the published NP1 genes of GenBank. The gene was amplified by PCR,and then cloned and sequenced. The gene was analyzed by bioinformatics methods. The results showed that the content of random coil in the secondary structure was high(51. 77%). The protein of NP1 contained zero transmembrane regions,one glycosylation sites,thirty phosphorylation sites and six B cell antigen epitopes. These antigen epitopes were at the protein amino acid 7—14,27—50,59—73, 85—94,102—108,151—155 aera,respectively.关键词
猪博卡病毒/NP1基因/克隆/生物信息学分析Key words
porcine bocavirus/NP1 gene/cloning/bioinformatics analysis分类
农业科技引用本文复制引用
郑英帅,左奕,柴瑞影,戚妍,李雅楠,袁万哲,孙继国..猪博卡病毒NP1基因的克隆及其生物信息学分析[J].河南农业科学,2015,(3):148-151,4.
