河南农业科学Issue(4)：49-51,3.DOI:10.15933/j.cnki.1004-3268.2015.04.011
低温预处理和聚乙二醇处理对小麦花药出愈率的影响
Effects of Low Temperature Pretreatment and PEG on Callus Induction Rate of Wheat Anther
摘要
Abstract
Taking the wheat lines bred by Henan Institute of Education as tested materials and C17 as the basic culture medium,the influence of different low temperature pretreatment times and different PEG concentrations added in the medium on wheat anther callus rate was studied. The results showed that the callus rate of wheat anther was the highest(2. 108% ) when it was pretreated by low temperature for 4 d, which had good stability(CV = 0. 067). On the other hand,adding 80—120 mg / L of PEG in the medium could significantly increase the callus rate of wheat anther,the effect of 100 mg / L PEG being the best.关键词
低温预处理/聚乙二醇/小麦花药/出愈率Key words
low temperature pretreatment/PEG/wheat anther/callus induction rate分类
农业科技引用本文复制引用
杨雪,刘春雷,王丁,张丽琴,王世杰,常慧萍,邢文会,付瑞敏..低温预处理和聚乙二醇处理对小麦花药出愈率的影响[J].河南农业科学,2015,(4):49-51,3.基金项目
国家自然科学基金项目(31071413) （31071413）
河南省科技攻关重点项目(122102110189) （122102110189）
河南省自然科学研究项目(2011B210002) （2011B210002）
河南省高等学校重点科研项目(15A210020) （15A210020）
