河南农业科学Issue(5)：53-57,5.
甘蓝型油菜小孢子培养技术优化
Optimization of Microspore Culture Techniques of Brassica napus L.
摘要
Abstract
In order to optimize microspore culture techniques of Brassica napus L. and improve the embryo induction rate and seedling rate, the N14009 was used as experimental material to culture microspores. During the research,the effects on microspore culture were studied by different temperature pretreatment,concentration of activated carbon,medium type and agar concentration and concentration of colchicine . The results showed that:It got a good result by 4℃ and 32 . 5℃ temperature pretreatments for one day each,and the embryo induction rate was 4. 23 embryos/bud;When the concentration of activated carbon was 0. 5 g/L,the embryo induction rate reached the maximum,4. 23 embryos/bud;In addition,the seedling rate reached 51. 16% and 46. 67% in the MS and B5 media both with 12 g/L of agar, respectively. The regenerated plants were turned to MS that had different concentration of colchicine, the doubling efficiency of N14009 reached 43 . 47% and the seedling rate reached 76 . 67%, when the concentration of colchicine was 80 mg/L.关键词
甘蓝型油菜/小孢子培养/胚诱导率/成苗率/加倍效率Key words
Brassica napus L./microspore culture/embryo induction rate/seedling rate/doubling rate分类
农业科技引用本文复制引用
谭少敏,胡靖锋,杨红丽,徐学忠,鄢波,陈倩,施敏,和江明..甘蓝型油菜小孢子培养技术优化[J].河南农业科学,2015,(5):53-57,5.基金项目
农业部公益性行业专项(201003029) （201003029）
云南省科技厅重点新产品开发计划项目(2012BB017) （2012BB017）
云南省农业厅蔬菜新品种协作项目[云财农(2012)58号] （2012）
云南省科技厅科技富民强县计划(2014EB0330) （2014EB0330）
云南省科技厅国际合作项目(2014IA016) （2014IA016）
