河南农业科学Issue(6)：137-141,5.
4株鸽源新城疫病毒的分离鉴定及其全基因组序列分析
Isolation,Identification and Whole Genome Sequence Analysis of Four Newcastle Disease Virus Strains in Pigeons
摘要
Abstract
In order to investigate genetic ancestry and amino acids characteristic changes of four pigeon paramyxovirus type I ( PPMV-1 ) isolates of genotype Ⅵ PPMV-1 genome sequence, full-length genome sequences of four Newcastle disease virus( NDV) strains isolated from pigeons between 2007 and 2008 in Jiangsu province were determined.Our results showed that all these four isolates, according to the phylogenetic tree, belonged to the genotype Ⅵb. In addition, the four isolates were more related to European strains in subgroup EU/re than to Chinese strains isolated. Predicted amino acid sequences analysis among these strains, along with many reference sequences of NDV in GenBank, revealed that there was an unique mutation of amino acid for NDV strains of genotype Ⅵ of pigeons in nucleocapsid protein( NP ) , phosphoprotein ( P ) , haemagglutinin-neuraminidase ( HN ) protein and large protein ( L ) , respectively.关键词
新城疫病毒/鸽/基因Ⅵ型/全基因序列Key words
Newcastle disease virus/pigeon/genotype Ⅵ/complete genome sequence分类
农业科技引用本文复制引用
胡新岗,王伟伟,黄银云,吴双..4株鸽源新城疫病毒的分离鉴定及其全基因组序列分析[J].河南农业科学,2015,(6):137-141,5.基金项目
国家自然科学基金项目(31440083) （31440083）
江苏省高校“青蓝工程”项目 （）
江苏省科技支撑计划项目(BE2012366) （BE2012366）
江苏农牧科技职业学院科研项目(YB201006) （YB201006）
