中医药导报Issue(6)：8-10,3.
三七三醇皂苷对局灶性脑缺血再灌注大鼠GFAP mRNA和蛋白表达的影响
Effects of PTS on GFAP mRNA and Protein Expression in Rats with Following Focal Cerebral Ischemia- reperfusion
摘要
Abstract
Objective: To explore the dynamic changes in MPA2 GFAP mRNA and protein expression and the effect of PTS on it after focal ischemia reperfusion around the infarct area. Methods: The model of acute reperfusion injury after cere-bral ischemia in rat was made by middle cerebral artery occlusion. All animals were allowed 45 min ischemia and sacrificed after different durations of reperfusion (1, 7, 14, 21 and 28 days). The expression of MPA2 GFAP mRNA and protein around the infarct area in the rat brain, and the effect of PTS on it were observed by PCR and western Results: The mRNA and protein expression of MPA2 in the whole durations, the PTS groups was higher than that of normal sham and N.S groups (P>0.05). In contrast, the mRNA and protein expression of GFAP increased remarkably as compared with the normal sham and N.S groups in 1, 7, 14, 21 and 28 days after reperfusion (P<0.05). Conclusion: PTS can increase the expression of GFAP. PTS could enhance the level of NSC proliferation after middle cerebral artery occlusion reperfusion.关键词
三七三醇皂苷/脑缺血再灌注/微管相关蛋白/胶质纤维酸性蛋白/大鼠Key words
PTS/CerebralIschemia-reperfusion/MPA2/GFAP/Rat分类
医药卫生引用本文复制引用
张利军,白宇,侯郁青,程记伟,陈黎佶..三七三醇皂苷对局灶性脑缺血再灌注大鼠GFAP mRNA和蛋白表达的影响[J].中医药导报,2014,(6):8-10,3.基金项目
国家中医药管理局中医药重点学科-中医全科基金项目（2013QK10）；上海市教委预算内资助项目 （）
