湖泊科学Issue(1)：113-118,6.
河蟹养殖池塘微囊藻水华毒性及其光合作用活性特征
Toxicity and photochemical activity of Microcystis bloom in crab cultivation pond
摘要
Abstract
In July and August of 2013, Microcystis bloom occurred in a crab pond which was located in Wuzhong district , the city of Suzhou.In the present study, single and double PCR amplification for microcystin synthetase gene were used to detect whether the Microcystis bloom was toxigenic or not.All PCR products from Microcystis bloom materials were positive.Meanwhile,high per-formance liquid chromatography technique were used to measure the microcystin concentration of Microcystis bloom, the results showed that intracellular microcystin concentration were 1.49 and 0.88 μg/L in July and August, and intracellular microcystin concentration were 0.75 and 1.09μg/L, respectively.In addition, Phyto-PAM was applied to measure photochemical activity of bloom-forming Microcystis.The value of maximum quantum yield Fv/Fmwere 0.48 and 0.44 in July and August, and the effective quantum yield ΦPSI were 0.38 and 0.32, respectively.These results suggested that bloom-forming Microcystis had higher potential growth, and the value of non-photochemical quenching were 0.28 and 0.36.From the view of characteristical parameters of rapid light response light curve, it demonstrated that the photochemical activity of bloom-forming Microcystis in July is higher than that in August.The experiment results from this study suggested that water in crab culture pond has suffered from Microcystis bloom and microcystin contamination may present potential threat to crab food safety .关键词
河蟹养殖池塘/微囊藻水华/微囊藻毒素/微囊藻光合作用活性/浮游植物荧光仪Key words
Crab pond/Microcystis bloom/microcystin/photochemical activity/Phyto-PAM phytoplankton analyzer引用本文复制引用
李大命,周军,唐晟凯,李旭光,林海,张彤晴,周刚..河蟹养殖池塘微囊藻水华毒性及其光合作用活性特征[J].湖泊科学,2015,(1):113-118,6.基金项目
江苏省青年科学基金项目（ BK2012488）和国家“十二五”子课题河蟹池塘高效生态养殖技术集成与示范项目（2012BAD25B07）联合资助 （）
