湖泊科学Issue(3)：527-534,8.
湖光岩玛珥湖春季浮游植物对溶解态氮的吸收∗
Uptake of dissolved nitrogen by phytoplankton in spring in Huguangyan Maar Lake
摘要
Abstract
Using 15 N stable isotope tracer technique by adopting the in-situ flask culture, the uptake rates of ammonium, nitrate and urea by phytoplankton community were measured in Huguangyan Maar Lake, in order to characterize the nitrogen absorption and the characteristics of absorption kinetics by phytoplankton community in spring in the lake. The results showed that there were 54 species ( including varieties and forms belonging to 7 phyla) of phytoplankton in the lake, mainly from the Cyanophyta, Bacilla-riophyta and Chlorophyta, which accounted for 44. 68%, 26. 70% and 19. 21%, respectively. Microcystis flos-aquae and M. aeruginosa were the absolute dominant species, and the dominance indexes were 0. 39 and 0. 28, respectively. Uptake rate of am-monium of phytoplankton community was the highest, about 5. 8 times of nitrate, 4. 2 times of urea, and accounted for 73. 3% of the total nitrogen uptake of the three kinds of dissolved nitrogen. The relative preference indices for ammonium, nitrate and urea were 2. 907, 0. 190 and 1. 192, respectively. Phytoplankton communities prefer uptaking ammonium, followed by urea, and final-ly nitrate. The turnover time for ammonium, nitrate and urea were 3. 72, 57. 03 and 9. 07 h, respectively. Nitrogen uptake kinet-ics of phytoplankton community agree with Michaelis-Menten equation. The maximum uptake rate was ammonium>urea>nitrate, and affinity (1/Ks) was nitrate>ammonium>urea. Phytoplankton community of the Huguangyan Maar Lake has a high absorption potential of ammonium, and a certain affinity to nitrate, therefore, the lake has the ability to use nitrate.关键词
湖光岩玛珥湖/浮游植物/稳定同位素/氮吸收/吸收动力学Key words
Huguangyan Maar Lake/phytoplankton/stable isotope/nitrogen uptake/absorption kinetics引用本文复制引用
张国维,李长玲,黄翔鹄,古滨河..湖光岩玛珥湖春季浮游植物对溶解态氮的吸收∗[J].湖泊科学,2015,(3):527-534,8.基金项目
广东省科技攻关项目(2010B060200011)和广东省自然基金项目(9152408801000003)联合资助 （2010B060200011）
