华侨大学学报（自然科学版）Issue(5)：538-542,5.DOI:10.11830/ISSN.1000-5013.2014.05.0537
白斑综合症病毒对对虾Caspase基因的调控
Regulation of Prawn Caspase Gene by White Spot Syndromic Virus
摘要
Abstract
The prawn Caspase gene was previously found to play important roles in protecting host from viral infection. In order to further delineate the transcriptional regulation of Caspase in response to viral infection,the Caspase promoter was biotin?labeled and bind to streptavidin?beads.Through pulldown assay the Caspase promoter was founded to interact with two white spot syndromic virus (WSSV)proteins Vp38 and Vp41B.Luciferase reporter assay revealed that Vp38 and Vp41B act as repressor and activator of Caspase transcription respectively.RNAi inhibition of Vp38 and Vp41B indi-cate the two WSSV proteins play roles on the regulation of prawn hymocyte apoptosis.These results provided insights in-to the immune mechanism of invertebrates,and will also be helpful for shrimp viral disease control.关键词
对虾白斑综合症病毒/Caspase基因/调控/凋亡Key words
white spot syndromic virus/Caspase gene/regulation/apoptosis分类
生物科学引用本文复制引用
金春英,林金清,王蔚..白斑综合症病毒对对虾Caspase基因的调控[J].华侨大学学报（自然科学版）,2014,(5):538-542,5.基金项目
国家自然科学基金资助项目（31272684）；国家海洋局海洋生物遗传资源重点实验室开放研究基金项目 （）
