化学工程Issue(5)：6-9,34,5.DOI:10.3969/j.issn.1005-9954.2014.05.002
多菌混合固态发酵产纤维素酶研究
Cellulase production by solid-state fermentation with multi-strains
摘要
Abstract
Cellulase production efficiency can be improved via the mixed culture solid-state fermentation.In order to obtain high cellulase activity,alkali-treated sugarcane bagasse supplemented with wheat bran was used as enzyme fermentation substrate.The response surface method was introduced to optimize the solid-state mixed-fermentation with Trichoderma reesei CICC40359 and Penicillium decumbens SMX.The results indicate that the filter paper enzyme activity has an maximum value of 101.825 FPU/g under the following conditions:temperature is 28 ℃;mass and volume ratio of material to water is 1 ∶2.5 g/mL;V(Penicillium )∶V(Trichoderma) =3 ∶1, m(bagasse)∶m(wheat bran)=2∶1 ,inoculum dose is 0.08 g/mL and fermentation time is 3 d,which provides a basis for the next optimization work.The fermentation liquor showed high sugar content when the enzyme activity was high.These results indicate that simultaneous enzyme and ethanol production may be an effective approach for the economic production of bioethanol.关键词
木霉/青霉/纤维素酶/固态发酵/混菌发酵Key words
trichoderma/penicillium/cellulose/solid-state fermentation/multi-strains fermentation分类
轻工纺织引用本文复制引用
刘云云,张宇,许敬亮,何敏超,庄新姝,袁振宏..多菌混合固态发酵产纤维素酶研究[J].化学工程,2014,(5):6-9,34,5.基金项目
国家自然科学基金资助项目（21176237，21306196）；广州市科技攻关项目（2013J4300026）；中国科学院广州能源所长创新基金 （）
