华西口腔医学杂志Issue(6)：537-541,5.DOI:10.7518/hxkq.2014.06.002
层层静电自组装构建载药种植体的研究
Construction of drug-loaded titanium implants via layer-by-layer electrostatic self-assembly
摘要
Abstract
Objective This study aims to construct a long-term, osteogenesis-targeting HU-308 drug delivery implant by the layer-by-layer electrostatic self-assembly (LBL) technique, and observe the features of its delayed release in vitro. Methods A heparin (Hep) and chitosan (Chi) multilayer was coated on pure titanium using the LBL technique, and the titanium implants were dipped into the solution to load HU-308. The amount of loaded drug and release rates were measured using a UV-Vis spectrophotometer. The relationships between the loading efficiency, release time, and multilayer films were evaluated. The morphology of all the multilayers was characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Results The results showed that the Hep/Chi self-assembly multilayer was gradually fabricated on the titanium surface. HU-308 was successfully loaded on the titanium implants. The amount of loaded drug increased with the amount of multilayer films, except in the T20 group. In vitro drug release study showed that drug release was more difficult in the system with thicker films, and large amounts of multilayer films decreased the release speed. Both SEM and AFM measurements showed typical LBL deposition of Hep and Chi. Conclusion The HU-308 drug delivery implant is successfully fabricated via LBL technology. It could provide sustained release of HU-308 over 30 d. This type of implant may provide a new possibility of promoting implant–bone osseointegration for osteoporotic patients.关键词
钛种植体/药物缓释/HU-308/层层静电自组装/肝素/壳聚糖Key words
titanium implant/drug delivery/HU-308/layer-by-layer electrostatic self-assembly/heparin/chitosan分类
医药卫生引用本文复制引用
徐倩,冯青,欧俊,孙红..层层静电自组装构建载药种植体的研究[J].华西口腔医学杂志,2014,(6):537-541,5.基金项目
广西医疗卫生重点科研课题基金资助项目 （）
