华西口腔医学杂志Issue(1)：6-10,5.DOI:10.7518/hxkq.2015.01.002
水蛭素对牙龈成纤维细胞碱性成纤维细胞生长因子和转化生长因子-β1表达的影响
Effects of hirudin on the expression of basic fibroblast growth factor and transforming growth factor-β1 in human gin-gival fibroblasts
摘要
Abstract
Objective This study aimed to investigate the effects of hirudin on the expression of transforming growth factor (TGF-β1) and basic fibroblast growth factor (bFGF) in human gingival fibroblasts (HGFs) in vitro, as well to explore its func-tion in the mechanism of gingival remodeling. Methods After culturing was performed with classic tissue-explant method, HGFs were derived from normal gingival and gingival hyperplasia tissues followed by orthodontic treatments with different concentrations of hirudin. The mRNA and protein expression levels of TGF-β1 and bFGF were respectively detected by real time quantity polymerase chain reaction and immunocytochemistry. Results Compared with normal HGFs, TGF-β1 expres-sion promoted collagen synthesis of fibroblasts, whereas bFGF collagen synthesis was decreased in hyperplasia HGFs without hirudin (P<0.05). Hirudin significantly upregulated the expression levels of bFGF but downregulated TGF-β1 in hyperplasia HGFs (P<0.05). Conclusion Orthodontic force may influence the balance of collagen synthesis and degradation in HGFs. Hirudin may modulate the balance of HGF collagen metabolism, thereby promoting gingival remodeling.关键词
水蛭素/人牙龈成纤维细胞/转化生长因子-β1/碱性成纤维细胞生长因子Key words
hirudin/human gingival fibroblasts/transforming growth factor-β1/basic fibroblast growth factor分类
生物科学引用本文复制引用
郑怡,禤坤,南澜,莫水学..水蛭素对牙龈成纤维细胞碱性成纤维细胞生长因子和转化生长因子-β1表达的影响[J].华西口腔医学杂志,2015,(1):6-10,5.基金项目
高等学校博士学科点专项科研基金新教师类基金资助项目 （）
