华西口腔医学杂志Issue(1)：16-20,5.DOI:10.7518/hxkq.2015.01.004
大鼠三叉神经痛模型中痛阈及胶质细胞源性神经营养因子的变化
Changes in pain threshold and glial cell line-derived neurotrophic factor in rat model of trigeminal neuralgia
摘要
Abstract
Objective This research aims to study the changes in pain threshold and glial cell line-derived neurotrophic factor (GDNF) in a Sprague Dawley (SD) rat model of trigeminal neuralgia. Methods A total of 36 male SD rats were ran-domly divided into three groups: operative, sham-operative, and control. In the operative group, a chronic constriction injury (CCI) was caused by placing loose chromic gut ligatures around the right infraorbital nerve (ION). In the sham-operative group, the right ION was subjected to the same procedure, but without ligation. In the control group, the right ION was not subjected to any treatment. The pain thresholds of the three groups were recorded at different times after the operation. The GDNF expression in each group was analyzed via immunohistochemical staining. Results An allodynia to mechanical sti-mulation in the region of the ligated ION was observed starting on the 2nd week after operation. Pain thresholds started to increase gradually from the 6th week and returned to the original level at the 10th to 12th week after operation. Cells that ex-pressed the GDNF markedly increased in number in the operative group with changes observed at different times. Conclusion We use chronic constriction injury to the infraorbital nerve (CCI–ION) to establish a trigeminal neuralgia-like animal model in SD rats. GDNF may play a role in regulating pain by promoting the restoration and regeneration of nerve fibers.关键词
胶质细胞源性神经营养因子/三叉神经痛/动物模型Key words
glial cell line-derived neurotrophic factor/trigeminal neuralgia/animal model分类
医药卫生引用本文复制引用
秦泗佳,张晓红,金海威,高璐,王福..大鼠三叉神经痛模型中痛阈及胶质细胞源性神经营养因子的变化[J].华西口腔医学杂志,2015,(1):16-20,5.基金项目
辽宁省自然科学基金计划资助项目 （）
