华西口腔医学杂志Issue(2)：187-191,5.DOI:10.7518/hxkq.2015.02.017
十肽对变异链球菌作用的差异蛋白质组学研究
Differential proteomics on synthetic antimicrobial decapeptide against Streptococcus mutans
摘要
Abstract
Objective To compare the protein profiles between decapeptide-treated and untreated planktonic cells of Streptococcus mutans (S.mutans) by differential proteomic analysis to determine and identify the key proteins.Methods In our previous study, we investigated decapeptide (KKVVFKVKFK–NH2), which was a novel adenosine monophosphate. Com-pared with other oral pathogens tested, decapeptide had a preferential antibacterial activity against S. mutans. It also inhibited S. mutans biofilm formation and reduced the one-day developed biofilm. In the present study, we first synthesized decapep-tide, and then compared the protein profiles between decapeptide-treated and untreated planktonic cells of S. mutans by two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. We also verified different expressions of key protein enolase in the protein level. Results The results showed that decapeptide altered the protein expression of planktonic S. mutans. These proteins were functionally involved in carbohydrate degradation by gly-colysis, protein folding, conjunction, transport, translation, adenosine triphosphate binding, protein binding, sequence-specific DNA binding, transcription factor activity, and two-component response regulator activity. Western blot results showed that enolase protein expression decreased obviously in decapeptide-treated cells of S. mutans. Conclusion The protein expression of S. mutans significantly changed after synthetic antimicrobial decapeptide treatment, suggesting that decapeptide may pre-sent a preferential effect on oral caries by changing the expression of certain key proteins, such as enolase protein.关键词
十肽/变异链球菌/差异蛋白质组学Key words
decapeptide/Streptococcus mutans/differential proteomics分类
医药卫生引用本文复制引用
刘奕,费伟,王艳君,牟雁东,吴红崑..十肽对变异链球菌作用的差异蛋白质组学研究[J].华西口腔医学杂志,2015,(2):187-191,5.基金项目
四川省科技支撑计划基金资助项目 （）
