海洋科学Issue(2)：71-75,5.DOI:10.11759/hykx20130502003
一株产琼胶酶海洋细菌的分离与鉴定
Isolation and identification of a bacterial strain producing agarase from sea water
摘要
Abstract
To obtain microbes for producing high activity agarase, a series of bacterial strains in sea water were isolated, screened and identified. By prescreening in plate cultivation and twice screening in liquid cultivation, a strain designated as G-5 was isolated from sea water that was taken from Zhoushan coastal areas in Zhejiang Prov-ince. The activity of agarase produced by G-5 could reach 413.8 U/mL. The colony of G-5 was milky white and semitransparent with a wet surface. The cell of G-5 was Gram negative and rod-shaped with a size of 0.58~0.69μm×1.50~2.26μm. The 16S rDNA sequence of G-5 was 98%homologous to 15 strains of Vibrio species in the da-tabase of NCBI. It could be concluded that the strain G-5 was a Vibrio species.关键词
琼胶酶/弧菌(Vibrio sp.)/分离与鉴定/16S rDNAKey words
Agarase/Vibrio sp./Isolation and Identification/16S rDNA分类
生物科学引用本文复制引用
梅建凤,李莎,茅鹤婷,王鸿,应国清..一株产琼胶酶海洋细菌的分离与鉴定[J].海洋科学,2014,(2):71-75,5.基金项目
浙江自然科学基金项目(LY13B020011) （LY13B020011）
