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温度和光径对微绿球藻生长及营养成分含量的影响

杜晓凤 邹宁 孙东红 常林 赵萍

海洋科学Issue(4):50-54,5.
海洋科学Issue(4):50-54,5.DOI:10.11759/hykx20120419002

温度和光径对微绿球藻生长及营养成分含量的影响

Effect of temperature and optical path on growth rate and accumulation of nutrients of Nannochloropsis sp

杜晓凤 1邹宁 1孙东红 1常林 1赵萍1

作者信息

  • 1. 鲁东大学 生命科学学院,山东 烟台 264025
  • 折叠

摘要

Abstract

The effect of temperature and optical path of flat plate glass reactors on growth rate and organics con-tents of Nannochloropsis sp. was investigated, using a range of optical paths from 5 to 20 cm. The results showed that the temperature had a significant effect on the growth of Nannochloropsis (P<0.05), and a very significant ef-fect on the contents of mutrients (P<0.01). The cell growth had a highest speed at 28℃, when the content of soluble protein reached the maximum and that of polysaccharides reached the minimum. The biomass yield reached the maximum at 30℃. The contents of lipid and chlorophyll decreased with the increase of temperature, while the con-tent of carotenoid increased with the increase of temperature. The optical path of photobioreactors had a very sig-nificant effect on the growth of Nannochloropsis (P<0.01). Specific growth rate, maximal cell density (107cell/mL) and yield per unit volume (mg /(L·d) ) decreased with increase in optical path. Yield per unit area (g /(m2·d) ) in-creased with increased optical path. The contents of lipid, soluble protein, and polysaccharides decreased with in-creased optical path, but the contents of chlorophyll and carotenoid increased with increased optical path. The in-crease trend of carotenoids was more obvious than that of chlorophyll. In summary, the optimal optical path deter-mines the optimal culture density, as well as yield per unit volume and the productivity of organics. Under our con-ditions the optimal optical path for culturing Nannochloropsis to accumulate lipid, protein and polysaccharide in plate reactors was 5 cm, the optimal optical path for accumulating pigments was 20 cm.

关键词

微绿球藻(Nannochloropsis oculata Hibberd)/温度/光径/蛋白质/多糖/总脂/色素

Key words

Nannochloropsis sp./temperature/optical path/protein/polysaccharide/lipid/pigments

分类

农业科技

引用本文复制引用

杜晓凤,邹宁,孙东红,常林,赵萍..温度和光径对微绿球藻生长及营养成分含量的影响[J].海洋科学,2014,(4):50-54,5.

海洋科学

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