海洋科学Issue(7)：20-26,7.DOI:10.11759/hykx20130722001
一株高效褐藻酸降解菌的筛选、鉴定及其发酵条件的优化
The screening, identification of alginate degrading bacteria and optimization of fermentation conditions
摘要
Abstract
A screening work was carried out to obtain a bacterium with high alginate lyase activity by using alginate as sole source of carbon. The strain was identified asAlteromonas based on physiology and biochemistry analysis, 16S rDNA sequence analysis, and was namedAlteromonassp.H4. It was shown that the optimal culture medium composition of producing alginate lyase was as follows (w/v): alginate 0.6%, yeast extracts 0.5%, peptone 0.25% and NaCl 3%. The optimized culture condition was as follows: culture temperature 28℃, initial pH value 7.5, inoculum size 1.5%(v/v) and optimal harvest time 48 h. The activity of alginate lyase was activated by Fe3+, which hasn’t been reported in other alginate lyase production strains, and the inhibitory effect by Cu2+ was up to 45.78%. Under optimized conditions, the alginate lyase activity of the Alteromonas sp. H4 was146.45 U/ml, which was increased by 39.4% than that before optimization.关键词
褐藻酸钠降解菌/筛选/鉴定/酶活优化Key words
alginate degrading bacteria/screening/identification/optimization分类
生物科学引用本文复制引用
侯士昌,温少红,唐志红,崔玉琳,秦松..一株高效褐藻酸降解菌的筛选、鉴定及其发酵条件的优化[J].海洋科学,2014,(7):20-26,7.基金项目
国家自然科学基金资助项目(41176144) （41176144）
海洋公益性行业科研经费资助专项(20120527) （20120527）
山东省自然科学基金项目(JQ200914) （JQ200914）
