海洋科学Issue(7)：27-33,7.DOI:10.11759/hykx20130608001
海南红树林根系土壤中可培养细菌的多样性分析
Diversity of culturable bacteria from the soil of root system of mangrove forest of Beigang island in Hainan Province
摘要
Abstract
To investigate the diversity of soil bacteria in the root system of mangrove forest and potential microbacteria sou-rces in Hainan Province, we collected ten soil samples from Beigang island in December 2012. The samples were treated by the plate dilution method to isolate culturable marine bacteria. The diversity of marine bacteria was examined based on 16S rRNA gene sequence analysis. Totally 70 representative strains were selected from 122 isolates, and they were further sequenced and subjected to phylogenetic analysis. The bacterial population was composed of 4 major lineages, Proteobacteria (66%, with a predominance of Gammaproteobacteria), Bacteroidetes (19%), Firmicutes (11%) and Actinobacteria (4%). These culturable bacterial isolates belonged to 30 genera and 47 species. There were 9 strains identified as potential novel species and 2 strains were identified as potential novel genus. This study indicated that there was high bacterial diversity in the soil of root system of mangrove forest of Beigang island and many isolates were the characteristic groups in mangrove forest.关键词
红树林/海洋细菌/多样性Key words
mangrove/marine bacteria/diversity分类
生物科学引用本文复制引用
孙静,王素英,张德超..海南红树林根系土壤中可培养细菌的多样性分析[J].海洋科学,2014,(7):27-33,7.基金项目
国家青年自然科学基金资助项目(31200005) （31200005）
中国科学院海洋研究所“一三五”专项资助项目(2012IO060101,2012IO060102,2012IO060103) （2012IO060101,2012IO060102,2012IO060103）
中国科学院海洋研究所知识创新领域前沿项目(Y12711101Q) （Y12711101Q）
留学人员科技活动项目择优资助项目(Y32808103N) （Y32808103N）
