海洋科学Issue(8)：26-33,8.DOI:10.11759/hykx20130818001
ICP-AES 测定海洋生物体中13种元素的微波消解条件优化
Optimization of microwave digestion conditions for 13 elem-ents in marine organisms by ICP-AES
摘要
Abstract
Herein, thirteen elements including Se, Fe, Mn, Zn, Ca, Mg, Sr, As, Cd, Cr, Cu, Pb and Ni in eight kinds of marine organisms were determined by using microwave digestion technique, and applying Inductively Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES). The conditions of microwave digestion were optimized through orthogonal experiment design. The optimum conditions were as follows: the volume ratio of HNO3-H2O2︰6︰1, digestion temperature: 170 ℃, and digestion time: 20 min. The correlation coefficients of linear regression equation for all elements were above 0.999. The recovery rate of the method was in the range of 92.0%~104.8%, and the relative standard deviations were between 0.30%and 2.55%. The detection limit of the method ranged from 0.0010μg/g to 0.0468μg/g. The results indicated that the optimized method was convent, sensitivity, accurate and precision, and also can be used for simultaneous multi-element detection. It was suitable for determination of these elements in marine organisms, and also provided scientific foundation for evaluation of safety of seafood.关键词
条件优化/微波消解/ICP-AES/测定Key words
Condition optimization/Microwave digestion/ICP-AES/Determination分类
化学化工引用本文复制引用
孙玲玲,宋金明,林强,孙萱,于颖..ICP-AES 测定海洋生物体中13种元素的微波消解条件优化[J].海洋科学,2014,(8):26-33,8.基金项目
国家自然科学基金资助资目(41306070) （41306070）
