海洋科学Issue(10)：63-69,7.DOI:10.11759/hykx20130925003
海洋生境刀鲚菌群PCR-DGGE指纹图谱构建及分析
Construction and analysis of PCR-DGGE gene fingerprint of bacterial community in Coilia nasus from sea
摘要
Abstract
In order to investigate the bacterial community structure of Coilia nasus both in vivo and in environment, the bacterial community in gill, intestinal wall, intestinal contents and the water environment of C. nasus was compared and analyzed using a culture-free 16S rDNA PCR-DGGE fingerprinting technology. The results showed that bacterial PCR amplifications could be better separated on DGGE using a denaturing gradient ranging from 35%to 55% at 50 V at 60℃ for 10h. The samples from water environment, gill, intestinal wall and intestinal contents showed 29, 24, 19, and 14 bands, respectively with different signal intensities, which might reflect the existence of rich bacteria species. The clustering analysis showed that no significant differences between repeated samples. The similarity was more than 80%. The sample from water environment and gill of C. nasus joined together to form a branch, with a similarity of 52%and the similarity between those from the intestinal wall and intestinal contents of C. nasus was 41%. The attached bacterial flora forming four samples were dominated by uncultured bacteria, including proteobacteria, actinobacteria, and firmicutes. Here, for the first time, we constructed the PCR-DGGE gene fingerprint for bacterial community in C. nasus from sea.关键词
PCR-DGGE/菌群/刀鲚(Coilia nasus)/PCAKey words
PCR-DGGE/bacterial community/Coilia nasus/PCA分类
农业科技引用本文复制引用
聂志娟,徐钢春,程起群,杜富宽,张勇,顾若波..海洋生境刀鲚菌群PCR-DGGE指纹图谱构建及分析[J].海洋科学,2014,(10):63-69,7.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(2013JBFT04) （2013JBFT04）
农业部东海与远洋渔业资源开发利用重点实验室开放课题 （）
国家科技支撑计划项目(2012BAD26B05) （2012BAD26B05）
公益性行业科研专项(201203065) （201203065）
