海洋科学Issue(11)：8-16,9.DOI:10.11759/hykx20130509003
地塞米松和过氧化氢对合浦珠母贝珍珠层结构及基质蛋白表达的影响
Effects of Dexamethasone and peroxide on the nacre struc-ture and expression level of matrix protein in Pinctada fucata
摘要
Abstract
Signaling pathways are thought to play critical roles in biomineralization, however, not much evidence has been revealed in Pinctada fucata so far. Herein, we have studied the relevance between these signaling path-ways and the matrix proteins through inhibiting and activating TGFβ and NF-КB signaling pathways by using dexamethasone and peroxide. Results show that TGFβand NF-кB signaling pathways had negative correlation with the growth of nacre, and they contributed to the regulation of crystal form and the crystallization rate of calcium carbonate. The expression levels of pf-smad3, pf-rel and Nacrein decreased synchronously after DEX treatment, while increased synchronously after H2O2 treatment. The results indicate that DEX and H2O2 might regulate the matrix proteins and affect nacre structure by inhibiting and activating TGFβ and NF-кB signaling pathways. The results also suggest that there might be cross-coupling between immune system and biomineralization system in Pinctada fucata, and the two systems might share the same signaling pathways like TGFβand NF-кB pathways.关键词
地塞米松/过氧化氢/免疫/生物矿化/合浦珠母贝(Pinctada fucata)Key words
dexamethasone/peroxide/immune/biomineralization/Pinctada fucata分类
海洋科学引用本文复制引用
陈鹏,苏境坦,孔玮,王洪钟,张贵友,谢莉萍,张荣庆..地塞米松和过氧化氢对合浦珠母贝珍珠层结构及基质蛋白表达的影响[J].海洋科学,2014,(11):8-16,9.基金项目
国家重点基础研究发展计划项目(973计划,2010CB126405) （973计划,2010CB126405）
