海洋科学Issue(11)：17-24,8.DOI:10.11759/hykx20131126001
杂色龙虾线粒体基因组全序列的测定与分析
Sequencing and analysis of the complete mitochondrial geno-me of Panulirus versicolor
摘要
Abstract
The complete sequence of Panulirus versicolor mitochondrial genome was determined by using conventional PCR and conserved primers walking approaches. The basic characteristics and gene arrangement of P. versicolor mito-chondrial genome were analyzed. Combined with the mitochondrial genome of P. stimposni and P. homarus, the mito-chondrial genes encoding polymorphic loci and gene mutation of Panulirus were also analyzed. The results show that the entire mitochondrial genome of P. versicolor is 15700 bp in length, consisting of 13 protein-coding genes, 22 tRNA genes and 2 rRNA genes and D-loop control area, which maintained the original arrangement of crustacean animal mito-chondrial genomes. The composition of P. versicolor mitochondrial DNA base has AT preference, with 67%A+T con-tent and the content of A, T, C, and G was 34.6%, 32.1%, 20.5,%and 12.8%respectively. The secondary structure of 18 tRNA and 2 rRNA of P. versicolor was predicted. The percentage of polymorphic loci of Panulirus mitochondrial genome in nad 2, nad 5 gene and D-loop control region is high, which are suitable for the phylogenetic relationships, intraspecific polymorphism and genetic differentiation research of Panulirus as molecular markers.关键词
杂色龙虾(Panulirus versicolor)/线粒体基因组/RNA二级结构/分子标记Key words
Panulirus versicolor/mitochondrial genome/RNA secondary structure/molecular markers分类
生物科学引用本文复制引用
刘皓,姚维,刘海情,刘楚吾,刘丽..杂色龙虾线粒体基因组全序列的测定与分析[J].海洋科学,2014,(11):17-24,8.基金项目
国家科技支撑计划资助项目(2007BAD29B03) （2007BAD29B03）
