海洋科学Issue(12)：1-7,7.DOI:10.11759/hykx20140106001
兼养对雨生红球藻细胞生长的促进作用及藻株差异性
The cell growth enhancement and difference of 4 strains of Haematococcus pluvialis under mixotrophic culture mode
摘要
Abstract
The general and different characteristics of cell growth, photosynthetic performance index (PIABS) of 4 strains of Haematococcus pluvialis under mixotrophic (light+sodium acetate) conditions were studied by using chlorophyll a fluorescence and the cell growth rate. The results show that mixotrophic cultivation could significantly improve specific growth rate and cell densities in H. pluvialis compared with phototrophic cultivation. Sodium acetate (NaAc) not only provided organic carbon substance to Haematococcus for heterotrophic growth but also changed its PIABS of phototrophic growth. Interestingly, PIABS in mixotrophic culture was obviously lower than that of autotrophic culture on the first day, but became significant higher than that of autotrophic culture during the later culture period. The cell growth in mixotro-phic cultivation could be further divided into heterotrophic growth and autotrophic growth, and the above analysis indi-cates that the fast growth of cells at the early culture stage of mixotrophic cultivation mainly came from its heterotrophic growth, and that at the late culture stage was mainly contributed by its autotrophic growth. Moreover, the adaptabilities to sodium acetate and the optimum concentrations for growth of H. pluvialis varied greatly within different strains. Strain H0 required a relative high NaAc concentrations and exhibited the fastest cell growth among the 4 tested strains, while strain H6 presented a low NaAc demands and exhibited the slowest cell growth. Above results indicate that mixotrophic culture seems to be an effective way to shorten the production cycle and to improve biomass of H. pluvialis in mass cul-ture with suitable NaAc concentration addition for each strain.关键词
雨生红球藻/乙酸钠/叶绿素荧光/混合营养/微藻培养Key words
Haematococcus pluvialis/sodium acetate/Chl a fluorescence/mixotrophic, microalgal cultivation分类
农业科技引用本文复制引用
龙元薷,刘建国,张立涛..兼养对雨生红球藻细胞生长的促进作用及藻株差异性[J].海洋科学,2014,(12):1-7,7.基金项目
国家重点基础研究发展计划项目(2011CB200904) （2011CB200904）
江苏省自然科学基金重点项目(BK2011009) （BK2011009）
